This web interface is intended to allow the community an easily accessible way to browse the scRNA-seq data from all 58,041 human CD34+ hematopoietic stem and progenitor cells (HSPCs) profiled from 1st trimester fetal hematopoiesis through 8th decade of life elderly hematopoiesis in the following publication:

The dynamics of hematopoiesis over the human lifespan

Li H, Cote PD, Kuoch M, Ezike J, Frenis K, Afanassiev A, Greenstreet L, Tanaka-Yano M, Tarantino G, Zhang S, Whangbo J, Butty VL, Moiso E, Falchetti M, Lu K, Connelly GG, Morris V, Wang D, Chen AF, Bianchi G, Daley GQ, Garg S, Liu D, Chou ST, Regev A, Lummertz da Rocha E, Schiebinger G, Rowe RG. Nature Methods. 2024

Please cite if this data contributes to your own study

In the UMAP tab, the following browsing options are available:

• Color individual cells by age, cell type cluster, or gene expression intensity

• If coloring cells by gene expression intensity, you can select an individual gene or multiple genes (separate each gene name with just a single space)

• You can also filter which age groups are displayed or which cell type clusters are displayed, and also if you want the UMAP embedding to cell cycle regression, or not.

• You can also download any plots that you make, any Seurat objects for specific analysis done on this web interface , or the full Seurat object for the entire dataset.

In the Violin tab, the following options are available when comparing gene expression between a Group A of cells and a Group B of cells:

• Gene expression comparisons can be done for a single gene, or a group of genes (separate each gene name with just a single space).

• For both Group A and Group B, one can select which human age and which hematopoietic cell type cluster to include in the analysis.

• For violin plots, you can choose to have the individual cells displayed, or not, and also choose to display the p-value from a Wilcoxon Rank-Sum test, or not.

Please note that gene symbols need to be entered in all capital letters.